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利用粳稻Kinmaze/籼稻DV85杂交后代单粒传衍生的81个F11家系所组成的重组自交系(RecombinantInbredLines,RILs)群体,研究水稻光合功能相关性状的数量性状基因座(QTL)。在水稻抽穗后7d测定叶片全氮含量(TLN)、叶绿素a/b比值(Chl.a∶b)和叶绿素含量(Chl)。共检测到6个QTL,各QTL的LOD值为2.66～4.81,贡献率为11.2%～29.6%,其中,在第1、2和11染色体上检测到3个与全氮含量相关的QTL,相应贡献率为17.3%、15.3%、13.7%;在第3和4染色体检测到2个与叶绿素a/b比值相关的QTL,贡献率为13.8%和29.6%;在第1染色体检测到1个与叶绿素含量相关的QTL,贡献率为11.2%。4个QTL为本研究新检测的基因座。有趣的是,控制叶绿素含量的qCC1位于第1染色体上RFLP标记C122附近,与已报道的NADH谷氨酸合成酶基因位置一致,而叶绿素合成始于谷氨酸,暗示该基因座与水稻光合功能关系极为密切。然而,对抽穗后30d叶绿素含量进行QTL分析,结果未检测到与其相关的QTL,表明控制叶绿素含量qCC1效应随水稻叶片的衰老而降低。
A population of 81 recombinant inbred lines (RILs) composed of 81 F11 lines derived from the japonica Kinmaze / Indica hybrids DV800 was used to study the quantitative trait loci (QTLs) of rice traits related to photosynthesis. The total nitrogen content (TLN), chlorophyll a / b ratio (Chl.a:b) and chlorophyll content (Chl) were measured 7 days after rice heading. A total of 6 QTLs were detected, with LOD values ranging from 2.66 to 4.81 for all QTLs and their contribution rates ranging from 11.2% to 29.6%. Three QTLs related to total nitrogen content were detected on chromosomes 1, 2 and 11, The contribution rates were 17.3%, 15.3% and 13.7%, respectively. Two QTLs related to the chlorophyll a / b ratio were detected on chromosomes 3 and 4, accounting for 13.8% and 29.6% of all the QTLs detected on chromosome 1 and 1 The QTL related to chlorophyll content contributed 11.2%. Four QTLs were newly detected loci in this study. Interestingly, qCC1, which controls chlorophyll content, is located near the RFLP marker C122 on chromosome 1 and is consistent with the reported NADH glutamate synthetase gene, whereas chlorophyll synthesis begins with glutamate, suggesting that this locus is associated with photosynthetic function in rice Very close relationship. However, the QTL analysis of chlorophyll content at 30 days after heading showed that no QTLs were detected, indicating that controlling chlorophyll content qCC1 decreased with senescence of rice leaves.