论文部分内容阅读
目的探讨硫化氢(hydrogen sulfide,H2S)与糖尿病肾病(diabetic nephropathy,DN)大鼠肾细胞外基质过度积聚的关系。方法 21只SD大鼠随机分成正常对照组(对照组)、糖尿病肾病组(DN组)、硫化氢干预组(DN+H2S组)。链脲佐菌素(STZ)法建立糖尿病大鼠模型,DN+H_2S组给予Na HS治疗8周,其他组给予同等剂量的0.9%生理盐水治疗。观察3组大鼠肾脏病理改变、Brd U阳性细胞和α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)分布差异。结果与对照组比较,DN组大鼠尿蛋白量、相对肾重均明显增多,病理呈典型的DN改变,Brd U阳性细胞比值、肾小球α-SMA表达和分布显著增多(P<0.01);应用NaHS治疗后,大鼠肾脏DN改变明显减轻(P<0.01),BrdU阳性细胞比值、肾小球α-SMA表达和分布与正常对照组比较,差异无统计学意义(P>0.05)。结论 H_2S可以抑制DN大鼠肌性成纤维细胞增殖,减少α-SMA表达和分布,减缓DN大鼠的肾小球细胞外基质积聚。
Objective To investigate the relationship between hydrogen sulfide (H2S) and renal extracellular matrix accumulation in diabetic nephropathy (DN) rats. Methods Twenty-one SD rats were randomly divided into normal control group (DN group), DN group (DN + H2S group). Diabetic rats were induced by streptozotocin (STZ), and treated with Na HS for 8 weeks in DN + H 2 S group and 0.9% saline in other groups. The pathological changes, the difference of BrdU positive cells and α-smooth muscle actin (α-SMA) were observed in 3 groups. Results Compared with the control group, the urinary protein and relative kidney weight of DN group were significantly increased, the typical pathological changes of DN, the BrdU positive cells ratio and the expression and distribution of glomerular α-SMA increased significantly (P <0.01) (P <0.01). The ratio of BrdU positive cells and the expression and distribution of glomerular α-SMA were not significantly different between the two groups (P> 0.05). Conclusion H_2S can inhibit the proliferation of DN fibroblasts, decrease the expression and distribution of α-SMA, and attenuate the glomerular extracellular matrix accumulation in DN rats.