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目的探索硝酸沉淀蛋白与微波消解法对血中元素测定的影响。方法采用5%硝酸沉淀蛋白及微波消解法分别处理全血样,并保证最终硝酸浓度基本相同;标准曲线采用标准加入法,以消除基质干扰。结果各元素检出限,硝酸沉淀蛋白法为0.5~39μg/L,微波消解法为0.5~32μg/L;相关系数,硝酸沉淀蛋白法除硒以外,其余均≥0.998,微波消解法除铬以外,其余均≥0.998;精密度,硝酸沉淀蛋白法为1.4%~4.8%,微波消解法为4.1%~49.2%;加标回收率,硝酸沉淀蛋白法除铝外,其余均在82.5%~113%范围内,微波消解法在82.0%~123%范围内。结论血中As、Pb、Cd、Cr、Zn的测定前处理宜采用硝酸沉淀蛋白法,Se、Al宜采用微波消解法,而Mn、Cu则两种前处理方法均适用。
Objective To explore the effect of nitric acid precipitated protein and microwave digestion on the determination of blood elements. Methods The whole blood sample was treated with 5% nitric acid precipitated protein and microwave digestion method respectively, and the final nitric acid concentration was basically the same. The standard curve was added by standard method to eliminate matrix interference. Results The detection limit of each element was 0.5 ~ 39μg / L by nitric acid precipitation protein method and 0.5 ~ 32μg / L by microwave digestion method. The correlation coefficients were all ≥0.998 except nitric acid precipitation protein method except for chromium , The rest were ≥0.998; precision, nitric acid precipitated protein was 1.4% ~ 4.8%, microwave digestion was 4.1% ~ 49.2%; spiked recoveries, nitric acid precipitated protein except aluminum, the rest were 82.5% ~ 113 % Range, microwave digestion method in the range of 82.0% ~ 123%. Conclusion Pretreatment of As, Pb, Cd, Cr and Zn in blood should adopt nitric acid precipitation protein method, Se and Al should adopt microwave digestion method, while Mn and Cu are suitable for both pretreatment methods.