[目的]比较喷雾和灌根施药方式下吡虫啉在烟草植株的吸收传导分布规律。[方法]进行温室盆栽试验,采用液相色谱-质谱联用方法测定施药不同时间烟草根、茎、上部叶、中部叶、下部叶中吡虫啉含量。[结果]灌根施药方式下吡虫啉能被烟草根部吸收并转运至植株各个部位,而喷雾施药在烟草叶片上原始沉积量较大,通过内吸作用传导至烟草其他部位的含量较低。[结论]灌根施药在烟草植株内输导分布更加均匀,持效期更好,喷雾施药能较好的作用于叶片部位,速效性更好。 [Objective] The purpose was to compare the absorption and transmission of imidacloprid in tobacco plants under spray and irrigation modes. [Method] The greenhouse pot experiments were conducted to determine the contents of imidacloprid in tobacco roots, stems, upper leaves, middle leaves and lower leaves at different time intervals by liquid chromatography-mass spectrometry. [Result] Imidacloprid was absorbed by root of tobacco and translocated to all parts of plant under the method of irrigation and root application. However, the initial deposition of tobacco powder on tobacco leaves was higher by spraying and lower to other parts of tobacco by systemic action. [Conclusion] The irrigation and rooting application in tobacco plants was more uniform and the persistence period was better. The spray application could better act on the leaf parts with better quick-acting effect.