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目的 :探讨人胎脑低分子肿瘤抑制物对白血病细胞的作用机制及其细胞内游离Ca2 +浓度 ([Ca2 +]i)的变化。方法 :通过超滤技术分段提取胎脑低分子抑瘤物 ,采用光镜、相差显微镜和流式细胞仪检测凋亡 ,Fura 2荧光负荷技术测 [Ca2 +]i。结果 :胎脑低分子抑瘤物可明显抑制K 5 6 2白血病细胞的增殖 ,作用 4h后可出现典型的凋亡改变。白血病细胞凋亡过程中 ,胞内 [Ca2 +]i明显升高。结论 :胎脑低分子抑瘤物可能是通过诱导白血病细胞凋亡而抑制其增殖 ,在白血病细胞凋亡过程中 [Ca2 +]i升高。
Objective : To explore the mechanism of action of human fetal brain low molecular tumor suppressor on leukemia cells and the change of intracellular free Ca2 + concentration ([Ca2 +]i). METHODS: The low-molecular-weight tumor suppressor of fetal brain was extracted by ultrafiltration technique. Apoptosis was detected by light microscopy, phase-contrast microscopy, and flow cytometry. Fura 2 fluorescence loading technique was used to measure [Ca2 +]i. Results : The low molecular weight tumor suppressor of fetal brain can significantly inhibit the proliferation of K 562 leukemia cells, and typical apoptosis changes can occur after 4 hours. During the apoptosis of leukemic cells, intracellular [Ca2 +]i was significantly increased. Conclusion : The low molecular weight tumor suppressor of fetal brain may inhibit the proliferation of leukemic cells by inducing apoptosis, and [Ca2 +]i increased during apoptosis of leukemic cells.