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作者对10例已经活组织检查证实为肺泡蛋白沉着症(PAP)和33例其他肺疾病(包括肺癌、假淋巴瘤、卡氏肺囊虫病、念珠菌病、曲霉肿、肺炎、放射性肺纤维化等)作对照,应用纤维支气管镜进行支气管灌洗。将洗出物加入福尔马林,离心沉淀15分钟,细胞沉淀成块,再用石腊包埋,进行病理切片。用苏木精和伊红、PAS和alcian blue染色。PAP的患者具有独特的组织学变化:①肺泡巨噬细胞极少;②当有肺泡巨噬细胞出现时,巨噬细胞中偶含嗜伊红颗粒,与PAP患者肺活检所见相同;③在弥漫性的嗜伊红的衬底中,有较大的伊红体,较红细胞小,但大小不一,多数在2～4μm的范围内;④蛋白类PAS染色占优势,而无明显的alcina blue染色。其它对照组
The authors evaluated 10 patients who had been confirmed by biopsy as pulmonary alveolar proteinosis (PAP) and 33 other pulmonary diseases including lung cancer, pseudolymphoma, Pneumocystis carinii disease, candidiasis, aspergilloma, pneumonia, radiation pulmonary fibrosis Etc.) as a control, the use of bronchoscopy for bronchial lavage. The eluate was added to formalin, centrifuged for 15 minutes, the cells were pelleted, and then paraffin embedded for pathological sections. Stain with hematoxylin and eosin, PAS and alcian blue. PAP patients have unique histological changes: ① very few alveolar macrophages; ② when there are alveolar macrophages, macrophages occasionally containing eosinophilic particles, and lung biopsy PAP seen the same; ③ In the Diffuse eugenics in the substrate, a larger eosin body, smaller than the red blood cells, but different sizes, mostly in the range of 2 ~ 4μm; ④ protein PAS staining dominant, but no significant alcina blue staining. Other control groups