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目的探讨补体C3a-C3a受体(C3aR)信号通路对正常儿童外周血Treg/Th17免疫平衡的影响,以期为进一步研究该通路在相关疾病中的作用提供实验依据。方法健康体检儿童17例,其中男性10例,女性7例,平均年龄6.8岁。收取空腹外周血3 ml,提取外周血单个核细胞(PBMC),分为3组:1)正常对照组;2)人重组C3a处理组(C3a组);3)人重组C3a及C3a受体拮抗剂处理组(C3a+C3aRA组)。经过培养后应用流式细胞术分别检测各组中Treg细胞和Th17细胞占CD4+T细胞的百分比,并比较各组间Treg/Th17的比值。结果与正常对照组比较,C3a组Th17细胞比例升高(P<0.01),Treg/Th17的比值明显降低(P<0.01),Treg细胞比例有下降趋势,但无明显统计学差异(P>0.05);与C3a组比较,C3a+C3aRA组Th17细胞比例降低(P<0.05),Treg/Th17的比值明显升高(P<0.05),Treg细胞比例有上升趋势,但无统计学差异(P>0.05);而C3a+C3aRA组较正常对照组Th17细胞、Treg细胞比例以及Treg/Th17的比值均无统计学差异(P>0.05)。结论 C3a-C3aR信号通路可能介导正常生理状态下儿童外周血中Th17细胞活化,同时参与调控正常儿童Treg/Th17细胞免疫平衡。
Objective To investigate the effect of complement C3a-C3a receptor (C3aR) signaling pathway on the immune balance of Treg / Th17 in peripheral blood of normal children, in order to provide experimental evidence for further study on the role of this pathway in related diseases. Methods 17 cases of healthy children, including 10 males and 7 females, with an average age of 6.8 years. Peripheral blood mononuclear cells (PBMC) were collected from 3 ml of peripheral blood mononuclear cells (PBMC) and divided into 3 groups: 1) normal control group; 2) human recombinant C3a group (C3a group); 3) human recombinant C3a and C3a receptor antagonists Agent treatment group (C3a + C3aRA group). After culture, the percentage of Treg cells and Th17 cells in CD4 + T cells in each group were detected by flow cytometry, and the ratio of Treg / Th17 in each group was compared. Results Compared with the normal control group, the proportion of Th17 cells in C3a group was significantly increased (P <0.01), the ratio of Treg / Th17 was significantly decreased (P <0.01), the proportion of Treg cells was decreased, but there was no significant difference (P> 0.05 ). Compared with C3a group, the proportion of Th17 cells in C3a + C3aRA group was decreased (P <0.05), the ratio of Treg / Th17 was significantly increased (P <0.05), and the proportion of Treg cells was increased but not significantly different 0.05). However, the ratio of Th17 cells, Treg cells and Treg / Th17 in C3a + C3aRA group were not significantly different (P> 0.05). Conclusion The C3a-C3aR signaling pathway may mediate the activation of Th17 cells in peripheral blood of children under normal physiological conditions and regulate the immune balance of Treg / Th17 cells in normal children.