制备了软骨肉瘤ｃＤＮＡ探针，峰长０．１至３．５Ｋｂ，并以此同骨肉瘤、软骨瘤、直肠粘液腺癌、食管鳞癌进行Ｎｏｒｔｈｅｒｎ杂交，结果前二者出现杂交带并以骨肉瘤为强。表明该探针能够区分原发性骨间叶性肿瘤和继发性骨癌，并为构建减数杂交文库打下了基础。 A cDNA probe for chondrosarcoma was prepared and its peak length was 0.1 to 3.5 Kb, which was used to perform Northern hybridization with osteosarcoma, chondroma, rectal mucinous adenocarcinoma, and esophageal squamous cell carcinoma. The results showed that the first two showed hybridization bands and flesh The tumor is strong. This indicates that the probe can distinguish between primary bone metastases and secondary bone cancers, and lays the foundation for the construction of a subtractive hybridization library.