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目的:观察新加良附方对移植性小鼠肝癌(H22)生长抑制作用及其对肿瘤组织中Bcl-2和Bax表达影响。方法:建立移植型H22动物模型,并将动物模型随机分模型对照、环磷酰胺(CTX)与新加良附方大、中、小剂量5组。新加良附大、中、小剂量组给药量分别为10g/kg、5g/kg和2.5g/kg;CTX组给药计量为17mg/kg;模型组给予等量无菌生理盐水。连续给药、给水12天处死模型小鼠分离肿瘤,检测肿瘤大小、称重计算肿瘤抑制率,并将肿瘤组织切片,免疫组化法检测Bcl-2和Bax基因。结果:新加良附大剂量组肿瘤抑制率为48.5%,与模型对照组比较,有统计学意义(P<0.01);新加良附大、中剂量可降低肿瘤组织中Bcl-2蛋白基因表达,升高Bax蛋白基因表达,与模型组比较,有显著性差异(P<0.01,P<0.05)。结论:新加良附方可抑制H22瘤体生长,且下调Bcl-2蛋白基因表达,上调Bax基因,提示新加良附方在抗肿瘤方面具有进一步深入研究价值。
Objective: To observe the inhibitory effect of Xinjialiangfuxian on the growth of transplanted mouse hepatoma (H22) and its effect on the expression of Bcl-2 and Bax in tumor tissue. Methods: Transplanted H22 animal model was established and the animal models were randomly divided into model control group, cyclophosphamide (CTX) and Xin Jia Liang Fu Fang large, medium and small doses of 5 groups. The dose of Xingalalian large, middle and small dose groups were 10g / kg, 5g / kg and 2.5g / kg respectively; the dosage of CTX group was 17mg / kg; the model group was given the same amount of sterile saline. The mice were sacrificed on the 12th day. The mice were sacrificed and the size of tumor was measured. The tumor inhibition rate was calculated by weighing. The tumor tissues were sectioned and the Bcl-2 and Bax genes were detected by immunohistochemistry. Results: The tumor inhibition rate of Xin Jia Liang Fu large dose group was 48.5%, which was significantly lower than that of the model control group (P <0.01); Xin Jia Liang Fu large and medium dose could reduce the expression of Bcl-2 protein Compared with the model group, the expression of Bax protein was significantly increased (P <0.01, P <0.05). Conclusion: SGL can inhibit the growth of H22 tumor, down-regulate the expression of Bcl-2 protein and up-regulate Bax gene, suggesting that SGL has further research value in anti-tumor.