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目的:研究雷公藤多苷对大鼠肺移植后的免疫排斥反应的抑制作用及对外周血细胞的影响。方法:随机选取100只SD大鼠分为空白组、对照组和治疗组,空白组20只大鼠,大鼠为正常大鼠,对照组共40只大鼠(其中20只为供体,20只为受体)和治疗组40只(其中20只为供体,20只为受体)大鼠均采用改良三袖套法吻合技术建立大鼠左肺原位移植的动物模型。移植手术后空白组和对照组采用生理盐水灌胃(1 mL/d),治疗组用雷公藤多苷(50 mg/kg·d)灌胃,连续灌胃5 d。观察各组大鼠的肺的大体形态及镜下的病理变化,确定排斥反应的等级,并测量各组大鼠外周血中T细胞活化率。结果:对照组大鼠病理改变较治疗组严重,治疗组排斥反应等级低于对照组的级别,差别有统计学意义(P<0.05);对照组的T细胞活化率高于治疗组,差异有统计学意义(P<0.01);对照组的活化率高于空白组,差异有统计学意义(P<0.05);治疗组的活化率于空白组相近,差异没有统计学意义(P=0.274)。结论:雷公藤多苷对大鼠肺移植后的排斥反应有抑制作用,其抑制机制之一可能是通过降低外周血T细胞活化率达到的。
Objective: To study the inhibitory effect of tripterygium glycosides on immune rejection after lung transplantation in rats and its effect on peripheral blood cells. Methods: A total of 100 SD rats were randomly divided into blank group, control group and treatment group, 20 rats in blank group, normal rats, 40 rats in control group (20 were donor, 20 Only for the receptor) and the treatment group 40 (20 for the donor, 20 for the receptor) rats were improved by three-cuff technique to establish the rat left lung orthotopic transplantation in animal models. After transplantation, the rats in the blank group and the control group were given gavage with normal saline (1 mL / d). The treatment group was given gavage with tripterygium glycosides (50 mg / kg · d) for 5 days. Observe the general morphology of the lungs and the pathological changes of the lungs of the rats in each group, determine the grade of rejection, and measure the activation rate of T cells in peripheral blood of rats in each group. Results: The pathological changes of the rats in the control group were more serious than those in the treatment group. The levels of rejection in the treatment group were lower than those in the control group (P <0.05). The T cell activation rate in the control group was higher than that in the treatment group (P <0.01). The activation rate of the control group was higher than that of the blank group, the difference was statistically significant (P <0.05). The activation rate of the treatment group was similar to that of the blank group, with no significant difference (P = 0.274) . CONCLUSION: Tripterygium glycosides inhibit the rejection after lung transplantation in rats, and one of the mechanisms may be through decreasing the rate of T cell activation in peripheral blood.