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为探讨流式细胞术(FCM)S期分数(S%)作为子宫内膜癌预后因素的意义,1984～1995年土耳其Istanbul大学Cerrahpasa医学院妇产科对80例宫内膜癌进行研究。标本取自切除的子宫或刮宫标本。标本组织石蜡块切成5～10μm厚组织片(含肿瘤组织)用Hedley改良法进行FCM分析。每个标本至少计数10万个细胞,变异系数<10%,非整倍体是组方图中第二个可辨峰,DNA指数(DI)为非整倍体平
To investigate the significance of flow cytometry (FCM) S-score as a prognostic factor for endometrial cancer, 80 cases of endometrial cancer were studied in the gynecological department of Cerrahpasa Medical College, Istanbul University, Turkey from 1984 to 1995. Specimens were taken from excised uterine or curettage specimens. The paraffin blocks of the specimens were cut into 5 to 10 μm thick tissue pieces (including tumor tissues) for FCM analysis using the Hedley modified method. Each specimen counted at least 100,000 cells with a coefficient of variation of <10%. Aneuploidy was the second identified peak in the panel plot and the DNA index (DI) was aneuploid