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目的：了解内皮素（ＥＴ）在蛛网膜下腔出血后急性及迟发性脑血管痉挛（ＣＶＳ）的病理生理过程中所起的作用。方法：本产验使用和成年日本长耳大白兔２１只，雌雄不限，体重在２．５～３．５ｋｇ。随机分为蛛网膜下腔出血３０ｍｉｎ，３ｄ，５ｄ三个实验组，每组７只，以术前做为对照组。采用枕大池穿剌取少量脑脊液后，一次注血法（０．５ｍｌ／ｋｇ）制成蛛网膜下腔出血动物模型。运用经颅多普勒技术（ＴＣＤ）观察兔基底动脉痉挛情况，在不同时间点再次穿剌枕大池取脑脊液，运用放射免疫技术定量测定脑脊液中ＥＴ浓度变化。结果：蛛网膜下腔出血（ＳＡＨ）后３０ｍｉｎ、３ｄ、５ｄＴＣＤ检查显示，兔基底动脉处于痉挛状态。ＥＴ浓度在ＳＡＨ后３０ｍｉｎ时无明显改变。第３、５ｄ增高达术前２倍且有统计学意义。结论：ＥＴ在急性ＣＶＳ和迟发性ＣＶＳ中的作用是不同的。ＥＴ并末参与急性ＣＶＳ发生，ＥＴ的过度分泌可能参与迟发性ＣＶＳ的发生及发展过程
Objective: To investigate the role of endothelin (ET) in the pathophysiology of acute and late cerebral vasospasm (CVS) after subarachnoid hemorrhage. Methods: This production test and adult Japanese long-eared rabbits 21, male or female, body weight 2.5 ~ 3.5kg. Randomly divided into subarachnoid hemorrhage 30min, 3d, 5d three experimental groups, each group of 7, preoperatively as a control group. After taking a small amount of cerebrospinal fluid through the large pool of puncture, a single injection of blood (0.5ml / kg) was made into animal models of subarachnoid hemorrhage. Transcranial Doppler (TCD) was used to observe the basilar artery spasm in rabbits. The cerebrospinal fluid (CSF) was again punctured at different time points and radioimmunoassay was used to quantitatively determine the concentration of ET in cerebrospinal fluid. Results: 30 min, 3 d and 5 d after the subarachnoid hemorrhage (SAH) showed that the rabbit basilar artery was in spasticity. ET concentration at 30min after SAH no significant change. The first 3,5d increased up to 2 times preoperative and statistically significant. Conclusion: The effects of ET on acute CVS and delayed CVS are different. ET is not involved in the occurrence of acute CVS, ET excessive secretion may be involved in the occurrence and development of delayed CVS