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目的:寻找鱼腥草与其易淆品百部还魂的鉴别特征,建立有效的鉴别方法。方法:LMVP(叶形态-脉序图谱鉴别法),QAERM(定量分析评价鉴别中药品种真伪方法的可靠性的方法)。结果:两者脉序类型均为“不达缘柄生环结-顶聚脉序”。主要区别:鱼腥草叶表面有灰白色气孔小凸起,托叶-叶柄鞘的舌状凸起明显,一级脉7或5条,近中脉的一级脉的靠拢点在湾缺上方或上端,靠拢段平直,鲜叶具明显的鱼腥气;百部还魂叶表面不具灰白色气孔小凸起,托叶-叶柄鞘的舌状凸起不明显,一级脉5条,近中脉的一级脉的靠拢点在湾缺内,靠拢段弯曲,鲜叶无鱼腥气。同时以上述区别点为指标,鉴别结果的正确性(Ac)=100%;重复性为符合率(ARO)=100%,一致性强度(Kappa)=1.00。结论:建立的“叶形态-脉序图谱鉴别法”可准确鉴别鱼腥草及易淆品百部还魂。操作简便、快速、价廉、可靠性强。
Objective: To find out the distinguishing characteristics of Houttuynia cordata and its hundred confused products, establish an effective identification method. Methods: LMVP (leaf morphology - pulse sequence mapping method), QAERM (quantitative analysis to evaluate the authenticity of traditional Chinese medicine methods of authenticity method). Results: Both types of pulse sequences are “not up to the edge of the ring knot - top clustering pulse sequence”. The main difference: Houttuynia leaf surface with small pores stomatal bulges, stipules - petiole sheath tongue prominent, a pulse of 7 or 5, near the first pulse of the vein close to the point in the absence of the upper or upper Bay , Near the straight section of the flat, fresh leaves with obvious fishy fishy; hundred resurgence of the surface of the leaves do not gray stomata small bulge, stipules - petiole sheath tongue protrusion is not obvious, a vein 5, near the midrib level Pulse closer to the point of missing in the Bay, close to the curved section, no fresh fish leaves. At the same time, the above differences were used as indicators, and the correctness of the identification results was (Ac) = 100%. The repeatability was ARO = 100% and Kappa = 1.00. Conclusion: The established “Leaf morphology - Identification of pulse sequence pattern ” can accurately identify the Houttuynia and confusion 100 revive. Easy to operate, fast, inexpensive, reliable.