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用雄性大鼠72只(体重180～250 g),随机为6批。每批12只,分为2组:1.给药组(6只)腹腔1次注射D-半乳糖胺600 mg/kg;2.对照组(6只)腹腔注射等量生理盐水。6批大鼠分别在给药后第1、2、3、5、7和10天取材。将右侧甲状腺固定于Bouin液内,每批两组甲状腺,埋入同一蜡块,制成6μm厚的石蜡连续片。取相邻切片分别进行HE染色、PAS反应、棓花青明矾染色和免疫组织化学PAP法显示T_3和T_4,并对甲状腺滤泡上皮细胞内的胶体小滴数量、滤泡腔内T免疫反应物的含量,分别作半定量分析。左侧甲状腺立即投入液氮骤冷。每批两组大鼠的甲状腺冻结在同一冰托上,恒冷箱切片,厚8μm,进行组织
72 male rats (weighing 180-250 g) were randomized into 6 batches. Each group of 12 rats was divided into two groups: 1. The administration group (6) intraperitoneal injection of D-galactosamine 600 mg / kg; 2. The control group (6) intraperitoneal injection of equal amount of saline. Six batches of rats were drawn on days 1, 2, 3, 5, 7 and 10, respectively. The right thyroid gland fixed in Bouin solution, each group of two thyroid glands, buried in the same wax block, made of 6μm thick continuous paraffin. T-3 and T-4 were detected by HE staining, PAS reaction, alum staining and immunohistochemical PAP method respectively. The number of colloidal droplets in thyroid follicular epithelial cells, the number of T immunoreactive substances in follicular cavity Content, respectively, for semi-quantitative analysis. Left thyroid immediately put liquid nitrogen to quench. The thyroid glands of each group of two rats were frozen on the same ice tray and sliced in a constant temperature cold box with a thickness of 8 μm for tissue