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目的观察不同死亡原因大鼠尸体血液中红细胞溶血速度的变化规律,为死亡原因的法医学推断提供新思路。方法 40只SD大鼠随机分为4组,分别以断髓、置于99%CO的空间、高坠、勒颈方式处死后,取各组大鼠右心室血液,于死后即刻(0h)、8h、16h、24h、32h、40h、48h、56h、64h、72h,采用显微镜数码图像法进行全血红细胞计数(CBC),并对组内和组间数据进行统计学比较分析。结果 4组血液红细胞数量在死后即刻至72h期间,均随时间的延长因溶血而减少。其中0~16h,各组溶血速度无明显差异(P>0.05);16~48h,速度加快,溶血速度以CO中毒组(88.50±25.99)%最快,其次为高坠(69.33±29.52)%和断髓组(48.78±3.17)%,机械性窒息组(41.90±9.61)%最慢,组间比较具有显著性差异(P<0.05);56h后,溶血速度再次减慢,至72h机械性窒息组仍有少量红细胞存在。结论 4组不同死因大鼠死后不同时间红细胞计数均减少,各组间差异具有统计学意义,其变化特征可为死亡原因的推断提供新的思路。
Objective To observe the changes of erythrocyte hemolysis velocity in dead cadaver blood of rats with different causes of death and provide new ideas for the forensic deduction of the cause of death. Methods Forty Sprague-Dawley rats were randomly divided into four groups. Each group was divided into four groups: the broken heart, the space of 99% CO, The whole blood red blood cell count (CBC) was measured by microscope digital image method. The intra-and inter-group data were statistically analyzed. Results The number of erythrocytes in the 4 groups decreased from hemolysis with the prolongation of time to 72h. The rate of hemolysis was the fastest in CO poisoning group (88.50 ± 25.99)%, followed by high fall (69.33 ± 29.52)%, and the rate of hemolysis was (48.78 ± 3.17)%, and mechanical asphyxia group (41.90 ± 9.61)%, the difference was significant (P <0.05). After 56h, the hemolysis speed slowed down again, Asphyxia group still a small amount of red blood cells. Conclusion The erythrocyte count of rats in 4 groups with different deaths decreased at different times after the death, and the differences among the groups were statistically significant. The change characteristics of the 3 groups could provide new ideas for the reason of death.