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通过聚合酶链式反应-限制性片段长度多态(PCR-RFLP)技术构建了红壤真菌的内转录间隔区(Internal Transcribed Spacer,ITS)rDNAs文库,比较分析了红壤地区典型森林土和农田土真菌群落结构对铝胁迫的响应,构建了6个土壤样品的真菌ITS rDNAs文库。从这6个克隆文库中随机挑取克隆进行了PCR-RFLP指纹图谱分析,共获得77个独特的真菌操作分类单元(OperationalTaxanomicalUints,OTUs)。对77个OTUs的代表性克隆测序并利用Blast工具进行分析(相似性95%~100%)。研究结果表明森林土壤和农田土壤样品中真菌类群79.2%(61)属于子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和球囊菌门(Glomeromycota),20.8%(16)属于未分类的簇(Unclassifiedfungi),其中担子菌门真菌在两种土壤中具有明显优势。物种多样性指数(Simpson、Shannon和Chao1)分析表明,铝胁迫降低了土壤真菌种群的多样性,农田土壤中真菌的种群多样性明显高于森林土壤。而且随着铝胁迫浓度的增加,担子菌门真菌在两种土壤中丰度明显下降,子囊菌门真菌的丰度显著上升,在高铝处理浓度处理的土壤中演变为优势种群。
The internal transcribed Spacer (rDNA) rDNAs library of red soil fungi was constructed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the typical forest soil and farmland soil fungi Community Structure Responses to Aluminum Stress, and 6 soil samples were constructed for ITS rDNAs library of fungi. Randomly picked clones from these six clones were analyzed by PCR-RFLP fingerprinting, and 77 unique Operational Taxonomy Units (OTUs) were obtained. Representative clones of 77 OTUs were sequenced and analyzed using the Blast tool (similarity 95% to 100%). The results showed that 79.2% (61) of fungal groups in forest soil and farmland soil samples belonged to Ascomycota, Basidiomycota and Glomeromycota, and 20.8% (16) belonged to unclassified clusters (Unclassifiedfungi), in which basidiomycotina has obvious advantages in both soils. Species diversity index (Simpson, Shannon and Chao1) analysis showed that aluminum stress reduced the diversity of soil fungi population, and the population diversity of fungi in farmland soil was significantly higher than that of forest soil. And with the increase of aluminum stress concentration, the abundance of Basidiomycotina fungi decreased obviously in both soils, the abundance of fungi in Ascomycota increased significantly and evolved into the dominant species in the soil treated with high concentration of aluminum.