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目的 :观察体外肿瘤抗原冲击致敏的白细胞介素 2 (IL 2 )基因修饰的巨噬细胞对肾癌小鼠的治疗效果并探讨其相关的免疫机理。方法 :通过重组腺病毒的介导 ,将IL 2基因转入新鲜分离的小鼠腹腔巨噬细胞 ,经肿瘤抗原冲击致敏后回输治疗原位肾癌小鼠 ,采用 4h5 1 Cr释放法检测脾脏NK和CTL活性。结果 :IL 2基因修饰的巨噬细胞经肿瘤抗原冲击后体内回输可使肾癌小鼠肺转移结节明显减少 ,存活期明显延长 ,40 %肾癌小鼠达到长期存活。治疗后荷瘤小鼠脾脏NK和CTL活性显著提高。结论 :IL 2基因修饰的巨噬细胞经肿瘤抗原冲击后自体回输是治疗肾癌的有效方法。
OBJECTIVE: To observe the therapeutic effect of interleukin 2 (IL 2) gene-modified macrophages sensitized by tumor antigen in vitro to mice with renal cell carcinoma and to explore the related immune mechanisms. Methods: IL2 gene was transfected into freshly isolated murine peritoneal macrophages by recombinant adenovirus mediated by recombinant adenovirus. The tumor cells were sensitized by tumor antigen and then transfused into orthotopic renal carcinoma mice. The 4h5 1 Cr release assay Spleen NK and CTL activity. Results: IL 2 gene-modified macrophages transfused in vivo after tumor antigen challenge significantly reduced the number of lung metastatic nodules and prolonged survival in 40% of renal cell carcinoma mice. The activity of NK and CTL in spleen of tumor-bearing mice significantly increased after treatment. CONCLUSION: Autologous transfusion of IL-2-modified macrophages after tumor antigen challenge is an effective treatment for renal cancer.