论文部分内容阅读
为了探讨不同水温和饥饿条件下鮸鱼抗氧化酶的变化规律,采用实验生态法分析了实验条件下鮸鱼(体质量234.46±52.85 g)肝脏中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力及其与体质量的相关分析。结果表明:饥饿导致实验鱼体质量下降,其中水温越高体质量下降越明显:30℃组下降(29.44±7.42)g,显著大于22℃组(9.06±1.47)g和13℃组(4.23±0.93)g。相同水温条件下,随着饥饿时间的延长,鮸鱼肝脏中SOD和CAT活力均出现明显变化,并且不同水温条件下变化各不相同:13℃组中,SOD和CAT活力均呈现先上升后下降的走势,在饥饿2 d时酶活力达到最高值;22℃组中,SOD和CAT从始至终均呈现逐渐下降的趋势;而在30℃高温组中,SOD与CAT活力变化趋势不完全一致,SOD活力变化较小,呈现轻微的先上升后下降趋势,同样是饥饿2 d时达到最大值,CAT活力总体表现为下降的趋势。同一饥饿时长不同温度处理组中,实验鱼肝脏中SOD和CAT活力同样存在显著差异(P<0.05):除了13℃组在鱼体饥饿2 d时CAT活力0.044±0.006 U/mgprot高于22℃组0.038±0.006 U/mgprot之外,SOD和CAT活力均是22℃组最高,30℃的高温组最低。本研究结果为鮸鱼的高效健康养殖提供技术参考。
In order to investigate the variation of antioxidant enzymes in anchovy under different water temperature and starvation conditions, the experimental ecological method was used to analyze the changes of superoxide dismutase (SOD) and catalase (CAT) in the liver of anchovy (body weight 234.46 ± 52.85 g) (CAT) vitality and its correlation with body weight. The results showed that the starvation resulted in the decrease of the quality of the experimental fish. The higher the temperature was, the lower the body weight was. The decrease of the body temperature was more obvious in the 30 ℃ group (29.44 ± 7.42) g, significantly higher than that in the 22 ℃ group (9.06 ± 1.47g) 0.93) g. Under the same water temperature, the activities of SOD and CAT in the liver of the catfish showed obvious changes with the prolongation of the starvation time, and the changes were different under different water temperature conditions. At 13 ℃, the activities of SOD and CAT increased first and then decreased , And reached the peak value at 2 d of starvation. In 22 ℃ group, the activities of SOD and CAT decreased gradually from the beginning to the end, while the activities of SOD and CAT in the 30 ℃ group were not consistent , The change of SOD activity was small, showing a slight first rising and then decreasing trend, also reached the maximum on the 2nd day of starvation, and the CAT activity showed a decreasing trend. There was also significant difference in the activity of SOD and CAT in the liver of experimental fish at the same duration of starvation (P <0.05) except for the CAT activity of 0.044 ± 0.006 U / mgprot at 22 ℃ Group 0.038 ± 0.006 U / mgprot, SOD and CAT activity were highest in 22 ℃ group, 30 ℃ in the lowest temperature group. The results of this study provide a technical reference for the efficient and healthy cultivation of anchovy.