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目的 :探讨脑热清 (NRQ)口服液的解热机制。方法 :复制家兔内毒素 (ET)性发热模型 ,观察NRQ对家兔体温的影响 ;用放射免疫法检测下丘脑和脑脊液 (CSF)中cAMP及腹中隔区AVP含量的变化。结果 :(1)NRQ +ET组的ΔT[(0 82± 0 0 8)℃ ]、TRI6(5 73± 0 0 9)、下丘脑cAMP含量 [(0 70± 0 5 0 )nmol/g]、CSF中cAMP含量 [(5 6 86± 1 34)nmol/L]及腹中隔区AVP含量 [(11 91± 3 4 7)ng/g],分别低于ET组的ΔT[(1 80± 0 16 )℃ ]、TRI6(11 31±0 2 0 )、下丘脑cAMP含量 [(1 35± 0 2 1)nmol/g]、CSF中cAMP含量 [(6 6 6 9± 1 82 )nmol/L]、腹中隔区AVP含量[(30 80± 9 5 9)ng/g],两者相比有显著差异 (P <0 0 1)。 (2 ) 4组的体温变化分别与下丘脑和CSF中cAMP以及腹中隔区AVP的变化呈正相关 (下丘脑 :r=0 899,P <0 0 5 ;CSF :r =0 991,P <0 0 1;AVP :r =0 972 ,P <0 0 1)。结论 :NRQ的解热机制可能是通过抑制下丘脑cAMP的生成与释放 ,同时通过促进腹中隔AVP释放两种途径发挥作用。
Objective : To investigate the antipyretic mechanism of Naoqingqing (NRQ) oral liquid. METHODS: Rabbit models of endotoxin (ET) fever were replicated. The effects of NRQ on body temperature in rabbits were observed. Radioimmunoassay was used to detect changes in cAMP and AVP content in ventral septal area of hypothalamus and cerebrospinal fluid (CSF). RESULTS: (1) ΔT [(0 82 ± 0 0 8) °C], TRI6 (5 73 ± 0 0 9), hypothalamic cAMP content [(0 70 ± 0 5 0) nmol/g] in the NRQ + ET group. The cAMP content in CSF [(5 6 86 ± 1 34) nmol/L] and abdominal septal area AVP content [(11 91 ± 3 4 7) ng/g] were lower than those in ET group [(1 80). ± 0 16 )°C ], TRI6(11 31±0 2 0 ), hypothalamic cAMP content [(1 35± 0 2 1) nmol/g], CSF cAMP content [(6 6 6 9± 1 82 )nmol /L], abdominal septal area AVP content [(30 80 ± 9 5 9) ng / g], there was a significant difference between the two (P <0 01). (2) Changes in body temperature in the 4 groups were positively correlated with cAMP in the hypothalamus and CSF and AVP in the ventral septal area (hypothalamus: r=0 899, P <0 05; CSF: r = 0 991, P < 0 0 1;AVP :r =0 972 ,P <0 0 1). Conclusion: The antipyretic mechanism of NRQ may be through inhibiting the production and release of cAMP in the hypothalamus, and at the same time, it can also play a role by promoting the release of AVP from the ventral septum.