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目的:探讨CO释放抑制剂锌卟啉Ⅸ(ZnPPⅨ)和NO释放抑制剂N-硝基-L-精氨酸甲酯(L-NAME)对大鼠阴茎组织中cGMP含量的影响。方法:180～220g Wistar大鼠30只,随机均分为3组(正常对照组,ZnPPⅨ组,L-NAME组),ZnPPⅨ组给予ZnPPⅨ[45μmol/(kg.d)],L-NAME组给予L-NAME[50mg/(kg.d)],正常对照组给予生理盐水[1ml/(kg.d)]。用药7d后断头处死大鼠,取阴茎制成匀浆。测定匀浆液中NOS、NO、CO、cGMP含量。结果:与对照组比较,应用CO释放抑制剂ZnPPⅨ后,大鼠阴茎组织中CO、NOS、NO、cGMP含量有一定程度的下降(P<0.05);应用NO释放抑制剂L-NAME后,大鼠阴茎组织中CO、NOS、NO、cGMP含量也较对照组有一定程度的下降(P<0.05)。结论:ZnPPⅨ与L-NAME能够降低大鼠阴茎组织中CO与NO的浓度,同时cGMP的含量也降低。
OBJECTIVE: To investigate the effect of the CO releasing inhibitor ZnPPⅨ and NO releasing inhibitor N-nitro-L-arginine methyl ester (L-NAME) on the cGMP content in rat penile tissue. Methods: Thirty Wistar rats (180-220 g) were randomly divided into three groups (normal control group, ZnPPⅨ group and L-NAME group), ZnPPⅨ group (45μmol / (kg · d) L-NAME [50mg / (kg · d)], normal control group was given saline [1ml / (kg · d)]. The rats were sacrificed after 7 days of decapitation, and the penis was made into a homogenate. Determination of homogenate in the NOS, NO, CO, cGMP content. Results: Compared with the control group, the content of CO, NOS, NO and cGMP in the penile tissue decreased to a certain extent (P <0.05) after the application of the CO releasing inhibitor ZnPPⅨ. After using NO releasing inhibitor L-NAME, The contents of CO, NOS, NO and cGMP in mouse penile tissue also decreased to a certain extent compared with the control group (P <0.05). Conclusion: ZnPPⅨ and L-NAME can reduce the concentration of CO and NO in the penile tissue of rats, meanwhile, the content of cGMP also decreases.