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目的：建立乙型肝炎病毒（ｈｅｐａｔｉｔｉｓＢｖｉｒｕｓ，ＨＢＶ）ＰｒｅＳ抗原检测方法。方法：表达与纯化了ＨＢＶＰｒｅＳ１／２抗原，并用纯化蛋白免疫家兔，利用纯化抗血清包被ＥＬＩＳＡ板，吸附病人血清中包含ＰｒｅＳ１／２抗原的ＨＢＶ病毒颗粒或病毒亚单位，特异吸附颗粒用抗ＨＢｓＡｇ的酶标单抗检测。结果：利用金属螯合柱层析的方法高度纯化了ＰｒｅＳ重组抗原，用复性的重组抗原免疫家兔３次，血清抗体滴度可达１∶５１．２万。利用纯化抗血清建立的ＰｒｅＳ１／２抗原测定方法具有较高的灵敏度与特异性。在３００份ＨＢｅＡｇ阳性与１９２份ＨＢｅＡｇ阴性血清中分别检测到２６０份（８７％）与１４２份（７４％）阳性血清。结论：初步结果表明，乙型肝炎病人血清中ＰｒｅＳ１／２抗原的检测与ＨＢｅＡｇ的状况无关
Objective: To establish a method for detecting PreS antigen of hepatitis B virus (HBV). Methods: The HBV PreS1 / 2 antigen was expressed and purified, and the rabbit was immunized with the purified protein. The ELISA plate was coated with the purified antiserum, and the HBV DNA or virus subunits of PreS1 / 2 antigen were adsorbed in the serum of patients. HBsAg ELISA monoclonal antibody detection. Results: PreS recombinant antigens were highly purified by metal chelate column chromatography. Rabbits were immunized three times with renatured recombinant antigens, and the titer of serum antibody was 1:51.2 thousand. PreS1 / 2 antigen assay using purified antiserum has high sensitivity and specificity. 260 (87%) and 142 (74%) positive sera were detected in 300 HBeAg-positive and 192 HBeAg-negative sera, respectively. CONCLUSIONS: The preliminary results show that the detection of PreS1 / 2 antigen in the serum of hepatitis B patients has nothing to do with the status of HBeAg