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以合成的含噁二唑杂环的罗丹明荧光染料(Rh-M)对Hela和U937细胞进行了活体染色实验.荧光显微镜下观察该荧光染料可以在5 min内进入Hela细胞,染色后的细胞发光面积较大,强度较高,Rh-M浓度在10-20μmol/L,染色15 min效果最佳.激光扫描共聚焦显微镜(Confocal laser scanning microscope,CLSM)下,浓度在20μmol/L染色15 min的Hela细胞,可以清晰看出染料在细胞质中均匀分布.流式细胞术((flow cytometry,FCM)检测U937细胞,表明Rh-M与肿瘤细胞的亲和能力强,在100μmol/L内荧光亮度与荧光染料浓度呈现增加趋势.
Hela and U937 cells were stained with rhodamine fluorescent dye (Rh-M) with synthetic oxadiazole heterocycle.It was observed under fluorescent microscope that the fluorescent dye could enter into Hela cells within 5 min.The stained cells The results showed that Rh-M concentration of 10-20μmol / L and staining of 15min were the best when the concentration of Rh-M was 15 min after confocal laser scanning microscope (CLSM) Of Hela cells, we can see clearly that the dye distributes evenly in the cytoplasm.Using flow cytometry (FCM) to detect U937 cells, it shows that Rh-M has strong affinity with tumor cells, and fluorescence intensity at 100μmol / L And fluorescent dye concentration showed an increasing trend.