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目的调查吉林地区流感病毒流行株对金刚烷胺的耐药情况。方法提取2005-2008年吉林地区流感流行季节分离的病毒流行株RNA,采用RT-PCR法扩增流感病毒的M2基因片断,检测病毒核酸测序并用生物信息软件分析与耐药性有关的氨基酸位点。结果16株流感病毒全部对金刚烷胺类药耐药,耐药株变异表现为31位丝氨基酸被天冬酰胺置换。结论金刚烷胺类药已不适用于流行性感冒的治疗,应进一步开展抗流感病毒耐药性的监测;同时应积极开展流感病毒对神经氨酸酶抑制剂类药物的监测。
Objective To investigate the resistance of amantadine to influenza pandemic strain in Jilin area. Methods RNA was isolated from the epidemic season of influenza in Jilin area during 2005-2008. The M2 gene fragment of influenza virus was amplified by RT-PCR, and the viral nucleic acid sequence was detected. Bioinformatics software was used to analyze the amino acid residues related to drug resistance . Results All the 16 influenza viruses were resistant to amantadine, and the variation of resistant strains showed that the 31 amino acids were replaced by asparagine. Conclusion Amantadine drugs are not suitable for the treatment of influenza, and should further develop anti-influenza virus drug resistance monitoring; at the same time should actively carry out influenza virus on neuraminidase inhibitors drugs monitoring.