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为明确岩木瓜Ficus tsiangii Merr.exCorner的杀虫活性成分及杀虫活性,利用活性跟踪分离技术,通过硅胶柱层析、薄层层析及葡聚糖凝胶等手段对岩木瓜茎和根皮的化学成分进行了分离、纯化,结合化合物理化性质和波谱数据对其结构进行了鉴定。结果表明,从岩木瓜中分离得到4个生物活性化合物,分别为β-香树脂醇乙酸酯、蒲公英赛酮、柯依利素及齐墩果酸。利用胃毒法测定了这些化合物(500μg/g糖)对家蝇Musca domestica成虫的生物活性,其中蒲公英赛酮处理试虫48h后的校正死亡率达到83.33%,表现出较强杀虫活性,并进一步测定了其对试虫的毒力。结果表明,蒲公英赛酮处理家蝇成虫48h后表现出较强毒力,其LC50值为89.82μg/g糖,略低于对照药剂鱼藤酮毒力(LC50值为67.58μg/g糖)。因此,蒲公英赛酮值得进一步深入研究。
In order to clarify the insecticidal active ingredients and insecticidal activity of Ficus tsiangii Merr.exCorner, using the active-tracing separation technique, the stems and root barks of A. papaya were separated by silica gel column chromatography, TLC and Sephadex The chemical constituents were separated and purified. Their structures were identified based on the physicochemical properties and spectral data of the compounds. The results showed that four bioactive compounds were isolated from the rock papaya, which were β-amyrin acetate, dandelion ketone, oxylysine and oleanolic acid. The bioactivities of these compounds (500μg / g sugar) against Musca domestica adults were determined by gastrovirus method. The corrected mortality of the testis reached 83.33% after 48 hours of treatment with dandelion, and showed strong insecticidal activity Its toxicity to test insects was further determined. The results showed that Dandelion treated with housefly exhibited strong virulence 48 h after LC. The LC50 value was 89.82 μg / g, slightly lower than that of the control agent rotenone (LC50 = 67.58 μg / g). Therefore, dandelion ketone worth further study.