The main purpose of the present work is to discuss whether or not the collective flows in heavy-ion collision at the Fermi energy can be taken as a tool to investigate the cluster configuration in light nuclei. In practice, within an extended quantum molecular dynamics model, four α-clustering(linear chain, kite, square and tetrahedron)configurations of 160 are employed in the initialization, ~(16)O+~(16)O around the Fermi energy(40-60 MeV/nucleon)with impact parameter 1-3 fm are simulated, and the directed and elliptic flows are analyzed. It is found that collective flows are influenced by the different α-clustering configurations, and the directed flow of free protons is more sensitive to the initial cluster configuration than the elliptic flow. Nuclear reaction at the Fermi energy can be taken as a useful way to study cluster configuration in light nuclei. The main purpose of the present work is to discuss whether or not the collective flows in the heavy-ion collision at the fermi energy can be taken as a tool to investigate the cluster configuration in light nuclei. In practice, within an extended quantum molecular dynamics model , four α-clustering (linear chain, kite, square and tetrahedron) configurations of 160 are employed in the initialization, ~16 O + ~16 O around the Fermi energy 40-60 MeV / nucleon with impact parameter 1 -3 fm are simulated, and the directed and elliptic flows are influenced by the different α-clustering configurations, and the directed flow of free protons is more sensitive to the initial cluster configuration than the elliptic flow . Nuclear reaction at the Fermi energy can be taken as a useful way to study cluster configuration in light nuclei.