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应用柑桔黄龙病单克隆抗体，通过胶体金标记，检测病叶徒手切片，在４００ ×显微镜下观察，韧皮部病灶呈黑褐色斑点；用异硫氰荧光素标记二次抗体法检测病叶切片，在激光共焦扫描显微镜下观察，病叶韧皮部呈现白色荧光斑点．经扫描其特异荧光强度平均值，可作定量参考．按 Ｊａｇｏｕｅｉｘ 报道的黄龙病病原１６ｓ Ｄ Ｎ Ａ 序列，设计引物，用 Ｐ Ｃ Ｒ 技术检测病叶 Ｄ Ｎ Ａ，获得特异性扩增区带，约１１６０ｂｐ ，可作为诊断依据．
Application of citrus Huanglongbing monoclonal antibody, colloidal gold markers, the detection of diseased leaves by hand section, observed under 400 × microscope, phloem lesions showed dark brown spots; with isothiocyanate-fluorescein-labeled secondary antibody method to detect diseased leaves slices, Under laser confocal scanning microscope, the diseased leaves phloem showed white fluorescent spots. After scanning the average value of its specific fluorescence intensity, can be used as a quantitative reference. According to the 16s D N A sequence of the pathogen of the yellow dragonfly disease reported by Jagoueix, a primer was designed and the D N A of the diseased leaf was detected by the P C R technique to obtain a specific amplification band of about 1160 bp, which can be used as a diagnosis basis.