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目的:探讨巨噬细胞抑制细胞因子-1(macrophage inhibitory cytokine-1,MIC-1)及尿激酶型纤溶酶原激活剂(urokinaseplasminogen activator,uPA)蛋白表达与骨肉瘤发生、发展的关系。方法:应用免疫组织化学SP法检测53例骨肉瘤和20例良性骨肿瘤组织中MIC-1及uPA蛋白表达。结果:53例骨肉瘤组织中MIC-1及uPA蛋白阳性表达率分别为58.5%和62.3%;20例良性骨肿瘤中MIC-1及uPA蛋白阳性表达率分别为10.0%和0,骨肉瘤组织和良性骨肿瘤组织MIC-1及uPA蛋白表达差异均有统计学意义(χ2=13.784及22.726,P均<0.05)。MIC-1、uPA蛋白表达与骨肉瘤组织Enneking分级、有无远端转移均密切相关。且二者蛋白表达呈正相关关系(γ=0.292,P<0.05)。结论:MIC-1和uPA在骨肉瘤发生、发展过程中起重要作用,MIC-1及uPA的联合检测有望成为骨肉瘤早期诊断和判断预后的分子指标之一。
Objective: To investigate the relationship between the expression of macrophage inhibitory cytokine-1 (MIC-1) and urokinase-type plasminogen activator (uPA) and the occurrence and development of osteosarcoma. Methods: The expressions of MIC-1 and uPA in 53 cases of osteosarcoma and 20 cases of benign bone tumor were detected by immunohistochemistry SP method. Results: The positive rates of MIC-1 and uPA in 53 osteosarcoma tissues were 58.5% and 62.3%, respectively. The positive rates of MIC-1 and uPA in 20 benign bone tumors were 10.0% and 0 respectively. There was significant difference in the expressions of MIC-1 and uPA protein in benign bone tumor (χ2 = 13.784 and 22.726, P <0.05). MIC-1, uPA protein expression and osteosarcoma Enneking classification, with or without distant metastasis are closely related. There was a positive correlation between them (γ = 0.292, P <0.05). Conclusions: MIC-1 and uPA play an important role in the development and progression of osteosarcoma. Combined detection of MIC-1 and uPA may become one of the molecular markers for the early diagnosis and prognosis of osteosarcoma.