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报道了用咖啡因加热休克抑制受精卵的第一极体的释放诱导皱纹盘鲍（Ｈａｌｉｏｔｉｓｄｉｓｃｕｓ ｈａｎｎａｉ Ｉｎｏ）多倍体的研究结果。皱纹盘鲍的卵在 ２１℃海水中受精，受精后 １０分钟开始处理，药物浓度分别为 ２．5ｍｍｏｌ／ Ｌ、５ｍｍｏｌ／ Ｌ、 １０ｍｍｏｌ／ Ｌ，热休克温度分别为 ２４℃、２６℃、２８℃，处理的持续时间为 １０分钟至 ３０分钟，共分 ５个时间段。结果表明，药物浓度５ｍｍｏｌ／ Ｌ和 １０ｍｍｏｌ／ Ｌ，处理温度２６℃，处理持续时间 １０～１５分钟，其三倍体诱导率可达６６．４％～７７．８％，综合评价指数为０．５７～０６１。在５ｍｍｏｌ／ Ｌ，２６℃条件下，处理持续时间 ３０分钟可以产生２．８％～３．６％的四倍体胚胎。
Reported the results of the study on the induction of polyploidy of Haliotis discus hannai Ino by caffeine heat shock inhibition of the release of the first polar body of fertilized eggs. The eggs of Haliotis discus hannai were fertilized in seawater at 21 ℃, and the treatment started at 10 min after fertilization. The drug concentrations were 2.5 mmol / L, 5 mmol / L and 10 mmol / L respectively. The heat shock temperatures were 24 ℃, 26 ℃ and 28 ℃ , The duration of treatment is 10 minutes to 30 minutes, is divided into 5 time periods. The results showed that the induction rate of the triploid reached 66.4% -77.8% and the comprehensive evaluation index was 0 at the concentration of 5mmol / L and 10mmol / L, the treatment temperature of 26 ℃ and the treatment duration of 10-15 minutes. 57 ~ 061. Under the condition of 5mmol / L, 26 ℃, the treatment duration of 30 minutes can produce 2.8% ~ 3.6% tetraploid embryos.