论文部分内容阅读
目的:探讨60min/d游泳运动对D-半乳糖致大鼠脑衰老和海马GAP-43表达的影响。方法:将42只SD大鼠随机分为:0.9%Nacl对照组(Na),衰老组(D),运动干预组(Ds),n=14。测定大脑皮质GSH-Px、SOD活性和MDA含量,Western blotting检测海马GAP-43蛋白表达。结果:D组较Na组行动迟缓,精神不振且嗜睡,皮毛无光泽、卷曲枯黄且伴有严重脱落等衰老体症明显;D组与Na组相比GSH-PX、SOD活性非常显著性降低(P<0.01),MDA含量非常显著升高(P<0.01);Ds组GSHPX活性显著性高于D组(P<0.05),SOD活性非常显著性高于D组(P<0.01),MDA含量非常显著性低于D组(P<0.01);Ds组和Na组GSH-PX、SOD活性和MDA含量均无显著性差异(P>0.05)。D组海马GAP-43蛋白表达量非常显著性低于Na组(P<0.01);Ds组海马GAP-43蛋白表达量非常显著性高于D组(P<0.01);Ds组海马GAP-43蛋白表达量显著性低于较Na组(P<0.05)。结论:(1)注射D-半乳糖(100mg/kg/d)6周,可以建立大鼠脑衰老模型。(2)在大鼠衰老过程中进行60min/d游泳运动干预可以延缓脑衰老,减缓海马GAP-43蛋白表达量的下调。
Objective: To investigate the effect of 60min / d swimming exercise on brain aging and hippocampal GAP-43 expression induced by D-galactose in rats. Methods: 42 SD rats were randomly divided into 0.9% Nacl control group (Na), aging group (D), exercise intervention group (Ds), n = 14. The GSH-Px, SOD activity and MDA content in cerebral cortex were determined. The expression of GAP-43 protein in hippocampus was detected by Western blotting. Results: Compared with na group, the activity of GSH-PX and SOD in group D was significantly lower than that in group Na (P <0.05). The activity of GSH-PX and SOD in group D was significantly lower than that in group Na (P <0.01). The content of MDA in the Ds group was significantly higher than that in the D group (P <0.05), the activity of SOD was significantly higher than that in the D group (P <0.01) (P <0.01). There was no significant difference in GSH-PX, SOD activity and MDA content between Ds group and Na group (P> 0.05). The protein expression of GAP-43 in hippocampus of D group was significantly lower than that of Na group (P <0.01). The expression of GAP-43 protein in hippocampus of Ds group was significantly higher than that of D group (P <0.01) Protein expression was significantly lower than the Na group (P <0.05). Conclusion: (1) The model of rat brain aging can be established by injecting D-galactose (100mg / kg / d) for 6 weeks. (2) The swimming time of 60min / d during the aging process of rats can delay brain aging and slow the down-regulation of GAP-43 protein expression in the hippocampus.