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为了从系统水平上阐明嗜热菌的耐热机制,并指导嗜热菌在工业生产上的进一步应用,以产甲烷菌的代谢网络作为研究对象,从研究代谢网络的拓扑结构以及高度紧密子网络出发,探索常温产甲烷菌Methanosarcina acetivorans(M.acetivorans)和嗜热产甲烷菌Methanopyrus kandleri(M.kandleri)之间的代谢网络的耐热性的差异。实验结果发现常温产甲烷菌M.acetivorans最紧密的9-核和嗜热产甲烷菌M.kandleri最紧密的7-核分别包含27和19个酶。其中嗜热产甲烷菌M.kandleri网络中最紧密的7-核被分成2个子网,一个子网中的酶节点刚好是2个产甲烷菌所共同的酶,而另一个子网络中的酶是嗜热产甲烷菌M.kandleri 7-核所特有的酶,这些特有的酶全部与酪氨酸的合成有关。
In order to elucidate the thermostable mechanism of thermophilic bacteria at the system level and to guide the further application of thermophilic bacteria in industrial production, the metabolic network of methanogens was taken as the research object, from the study of the topology of metabolic networks and the highly compact sub-networks To explore the difference in the heat resistance of the metabolic network between the methanogenic Methanosarcina acetivorans (M. acetivorans) and the thermophilic Methanopyrus kandleri (M. kandleri). The results showed that the most compact 9-nucleus and M. thermophilus M.kandleri, the most compact 7-nucleus of M. acetivorans at room temperature, contained 27 and 19 enzymes, respectively. The thermophilic methanogen M.kandleri network, the most compact 7-core is divided into two sub-networks, one sub-network in the enzyme node is exactly two methanogenic common enzymes, and another sub-network enzyme Is a thermophilic Methanobacterium M.kandleri 7-nuclear-specific enzymes, all of these unique enzymes and tyrosine synthesis.