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目的建立慢性饮水型氟中毒动物模型并观察其血清生化指标的改变。方法 48只Wistar大鼠随机分成4组,染氟组分别饮用含氟化钠(50、100、150mg/L)自来水,对照组饮用自来水;8周后麻醉处死,腹主动脉取血,测定血清钙、磷、尿酸及碱性磷酸酶(ALP)活性;用氟离子选择电极法测定各组大鼠血氟及骨氟(股骨)浓度。结果与对照组比较,低、中、高剂量染氟组大鼠血氟含量[分别为(0.175±0.025)、(0.230±0.036)、(0.313±0.042)μ/mL]与骨氟含量[分别为(893.628±67.748)、(1 001.111±34.121)、(1 248.152±77.131)μg/mg]显著升高(P<0.05);与对照组比较,低、中、高剂量染氟组大鼠血清钙含量[分别为(2.212±0.282)、(2.185±0.300)、(2.148±0.179)mmol/L]下降,血清磷[分别为(2.434±0.778)、(2.478±0.246)、(2.505±0.365)mmol/L]上升,差异均有统计学意义(P<0.05);与对照组比较,高剂量染氟组大鼠尿酸含量[(47.286±1.732)μmol/L]下降,ALP活性[(92.667±11.719)U/L]上升(P<0.01)。结论在排除其他代谢性骨病条件下,血清氟、钙、磷、尿酸和ALP可以作为地氟病辅助诊断依据。
Objective To establish a chronic drinking water fluorosis animal model and observe its serum biochemical changes. Methods Forty-eight Wistar rats were randomly divided into four groups. The rats in the fluoride group received drinking water containing sodium fluoride (50,100,150mg / L) respectively. The control group received tap water. After 8 weeks, the rats were anesthetized and sacrificed. Blood samples were obtained from the abdominal aorta. Calcium, phosphorus, uric acid and alkaline phosphatase (ALP) activity were measured. The concentrations of blood fluorine and bone fluorine (femur) in rats in each group were determined by fluoride ion selective electrode. Results Compared with the control group, the blood fluoride levels in the low, middle and high dose fluoride-treated rats [(0.175 ± 0.025), (0.230 ± 0.036) and (0.313 ± 0.042) μ / mL, respectively, (P <0.05). Compared with the control group, the levels of serum in the low, middle and high doses of fluoride-treated rats were significantly higher than that of the control group (893.628 ± 67.748, (1 001.111 ± 34.121), (1 248.152 ± 77.131) μg / (P <0.05). The levels of serum calcium [(2.212 ± 0.282, 2.185 ± 0.300, 2.148 ± 0.179 mmol / L, mmol / L], the difference was statistically significant (P <0.05). Compared with the control group, the content of uric acid in high dose fluoride group decreased (47.286 ± 1.732 μmol / L) and the ALP activity [(92.667 ± 11.719) U / L] increased (P <0.01). Conclusions The serum fluorine, calcium, phosphorus, uric acid and ALP can be used as auxiliary diagnosis of DXF under the condition of excluding other metabolic bone diseases.