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虽然甲醛甲酚(FC)在儿童齿科被广泛应用,但对其毒性、渗透性和感染牙髓的最终修复力仍有不同看法。作者旨在研究应用FC 5分钟后的乳酸脱氢酶(LDH)阴性区与核完整层及结构破坏层的相关性;并获得二层的组织破坏和被活组织取代的证据和时间;与使用氢氧化钙、生理盐水(NS)的结果相比较。作者选用8只年轻成年羊的51只切开,用FC或NS治疗者,经常规备洞、露髓、扩髓、冲洗、止血后,置FC或NS棉球5分钟,用氧化锌丁香油粘固粉(ZnOE)封闭。用氢氧化钙治疗者,行切髓术,置氢氧化钙,ZnOE封闭。酸蚀边缘釉质,在边缘釉质和牙骨质上涂一层,Uvio Bond,以防泄漏。3只未治疗牙作为LDH染色强度的对照。试验期为1、3、7、14和28天。处死羊,磨除釉质,根据摄片截根尖后,拔牙。36只牙放置于有数滴羧甲基纤维素的铝箔中,异成烷冷冻,液氮冷却,制作20μm冰冻切片。LDH活性培养是
Although formaldehyde cresol (FC) is widely used in pediatric dentistry, there is still a divergence of view regarding its toxicity, permeability, and ultimate repair of infected pulp. The aim of the study was to investigate the association of lactate dehydrogenase (LDH) negative regions with intact and structurally intact nuclei after 5 minutes of FC application and to obtain evidence and timing of tissue destruction and biopsy replacement in the second layer; Calcium hydroxide, saline (NS) compared to the results. The authors selected 51 cases of 8 young adult sheep incision, with FC or NS treatment, regular holes, demyelination, reaming, washing, bleeding, set FC or NS cotton balls for 5 minutes, with zinc oxide clove oil Cement powder (ZnOE) closed. Calcium hydroxide treatment, line incision, set calcium hydroxide, ZnOE closed. Erosion of the edge of the enamel, the edge of the enamel and cementum coated with a layer, Uvio Bond, to prevent leakage. Three untreated teeth served as control of LDH staining intensity. The test period is 1, 3, 7, 14 and 28 days. Sheep, abrade the enamel, according to the film after cutting the root tip, tooth extraction. 36 teeth placed in a few drops of carboxymethyl cellulose in aluminum foil, isobutane frozen, liquid nitrogen cooling, making 20μm frozen sections. LDH activity culture is