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目的研究更为简单易行效果好的日本血吸虫感染实验动物的方法。方法腹壁剪毛贴片法、腹壁拔毛贴片法、腹壁切口滴入法。结果血吸虫尾蚴通过腹部剪毛、腹部拔毛、腹部切口后感染家兔,获虫率分别为57.6%、59.1%、61.2%;血吸虫尾蚴通过腹部剪毛、腹部拔毛、腹部切口后感染小白鼠,获虫率分别为89.3%、94.6%、96.4%。结论家兔较脆弱,用腹壁切口滴入法易切口发炎导致死亡。虽获虫率较高,不提倡使用,使用腹壁拔毛贴片法最为可行;小白鼠生命力强,用腹壁切口滴入法感染获虫率最高,不易因切口发炎导致死亡,此法提倡使用。
Objective To study the more simple and effective method of infection of experimental animals with Schistosoma japonicum. Method abdominal shearing patch method, abdomen wall plucking patch method, abdominal incision drip method. Results Schistosoma japonicum cercariae infected rabbits by abdominal shearing, abdominal depilation and abdominal incision, and the infection rates were 57.6%, 59.1% and 61.2% respectively. The infected mice were infected with cercariae of Schistosoma japonicum through abdominal shearing, abdominal depilation and abdominal incision The worm rates were 89.3%, 94.6% and 96.4% respectively. Conclusion Rabbits are more fragile, with abdominal incision drip method easy to cut inflammation caused the death. Although the worm rate is higher, do not advocate the use of the abdomen wall plucking patch method is the most viable; mice with strong vitality, with abdominal incision inoculation infection by the highest rate of worm infection, not easy to cause inflammation due to incision death, this method is advocated.