建立了免疫亲和柱-液相色谱法快速测定面粉中黄曲霉毒素B_1的方法。样品经甲醇-水(80:20,v/v)提取,经过滤、沉淀(乙酸锌和亚铁氰化钾),通过免疫亲和柱净化,三氟乙酸和正己烷为衍生剂进行衍生,采用C_(18)柱为分离柱,乙腈:水(50:50,v/v)为流动相进行高效液相色谱分离分析。实验结果表明,黄曲霉毒素B_1线性范围(1~10)ng/m L,R2=0.9991,RSD=3.4%,检出限为0.1μg/kg,实际样品的黄曲霉毒素B_1回收率为81%~95%。该方法具有灵敏度高、回收率高、重现性好等优点,可用于面粉中黄曲霉毒素B_1的快速测定。 A rapid method for the determination of aflatoxin B1 in flour by immunoaffinity column and liquid chromatography was established. The sample was extracted with methanol-water (80:20, v / v), filtered, precipitated (zinc acetate and potassium ferrocyanide), purified by immunoaffinity column, derivatized with trifluoroacetic acid and n- The column was separated on a C_ (18) column with acetonitrile: water (50:50, v / v) as the mobile phase for HPLC analysis. The results showed that the linear range of aflatoxin B_1 was 1 ~ 10 ng / m L, R2 = 0.9991, RSD was 3.4%, and the detection limit was 0.1 μg / kg. The aflatoxin B 1 recovery was 81% ~ 95%. The method has the advantages of high sensitivity, high recovery, good reproducibility and the like, and can be used for the rapid determination of aflatoxin B1 in flour.