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目的:动态观察钠-钙交换体(NCX)mRNA和蛋白在氯化锂-匹罗卡品致模型大鼠海马CA1、CA3及齿状回区表达的变化,探讨其在癫发生发展中的作用。方法:用氯化锂-匹罗卡品制备癫动物模型;应用原位杂交和免疫组化技术检测各时间点NCX3mRNA和蛋白的表达。结果:急性期(6～24h)海马各区NCX3mRNA表达均随时间的延长逐渐减少;进入静止期各区表达趋向回升,慢性反复自发发作期(30、60d)各区表达又出现不同程度的两次下调。除致后6h大鼠海马各区的NCX3蛋白表达无明显变化外,NCX3蛋白变化趋势与NCX3mRNA基本一致。结论:NCX3表达下调可能通过增加神经元钙超载,改变海马神经元的兴奋性,促使癫发生。
OBJECTIVE: To observe the changes of NCX mRNA and protein expression in the hippocampal CA1, CA3 and dentate gyrus induced by lithium chloride-pilocarpine-induced rat model and to explore its role in the development of epilepsy effect. Methods: Epilepsy animal model was established by lithium-pilocarpine. The expression of NCX3 mRNA and protein at each time point was detected by in situ hybridization and immunohistochemistry. Results: The expression of NCX3mRNA in hippocampus of acute phase (6 ~ 24h) all decreased gradually with time prolonging. The expression of NCX3mRNA in each phase entering the quiescent phase tended to rebound, and the expression of NCX3 in each phase of chronic episode spontaneous seizure (30 and 60d) was reduced twice in varying degrees. Except 6h after the rat hippocampus NCX3 protein expression no significant change, NCX3 protein trends and NCX3mRNA basically the same. Conclusion: The down-regulation of NCX3 may induce epilepsy by increasing the calcium overload of neurons and changing the excitability of hippocampal neurons.