CD27抗原是一分子量为120-KDa的二聚体,属于富含半胱氨酸的细胞表面分子家族.过去CD27抗原一直被看做是使T细胞活化的抗原,后来研究表明,CD27还可在一些B细胞和自然杀伤细胞(NK细胞)上表达.文章探讨了CD27在NK细胞上的表达及其作用.用相应单抗加免疫磁珠的方法从外周血单核细胞(PBMC)中去除T,B和单核细胞,得到NK细胞,并以此为基础作为一系列实验. CD27 antigen is a dimer with a molecular weight of 120-KDa and belongs to a family of cell surface molecules rich in cysteine. In the past, the CD27 antigen was always regarded as an antigen that activates T cells. Later studies showed that CD27 was also found in Some B cells and natural killer cells (NK cells) .The expression of CD27 on NK cells and its role were explored.The T cells were removed from peripheral blood mononuclear cells (PBMC) by using the corresponding monoclonal antibodies and immunomagnetic beads , B and monocytes, NK cells were obtained and used as a basis for a series of experiments.