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通过荧光光谱和富立叶变换红外 (FT IR)光谱研究了阴离子型表面活性剂 -十二烷基硫酸钠 (SDS)与光系统Ⅱ (PSⅡ )的相互作用 .结果表明 ,PSⅡ表现出酪氨酸荧光的特性 .在PSⅡ蛋白质内部 ,存在着 2 32nm处的组分与酪氨酸之间以及这两种氨基酸残基与叶绿素a之间的能量传递 .SDS的存在会使这些能量传递以及PSⅡ中蛋白的骨架结构和酪氨酸残基的结构发生改变 ,而变化方式又明显受SDS在溶液中聚集状态的影响 .低于其临界胶束浓度(cmc)时 ,SDS会促进蛋白质中 2 32nm处的组分与酪氨酸之间的能量传递 ,并且使酪氨酸残基处于极性更小的环境 ;而大于cmc时 ,SDS却产生相反的效应 .但不同浓度的SDS均会抑制酪氨酸残基至叶绿素a的能量传递 .
The interaction between anionic surfactant sodium dodecyl sulfate (SDS) and photosystem Ⅱ (PSⅡ) was studied by fluorescence and Fourier transform infrared spectroscopy (FT IR) spectroscopy. The results showed that PS Ⅱ showed tyramine Acid fluorescence characteristics within the PSII protein, there is a component at 2 32nm and tyrosine and between these two amino acid residues and chlorophyll a energy transfer between the presence of SDS will make these energy transfer and PSII The skeletal structure and tyrosine residues in the protein structure change, and the mode of change is significantly affected by the aggregation state of the SDS in solution. Below its critical micelle concentration (cmc), SDS promotes the binding of the 2 32 nm And the tyrosine residues in the less polar environment; while more than cmc, SDS has the opposite effect, but different concentrations of SDS will inhibit the cheese The energy transfer from the amino acid residue to chlorophyll a.