论文部分内容阅读
目的分析G蛋白耦联受体5(Lgr5)在结肠癌侵袭转移过程中的作用及其意义。方法采用免疫组织化学法检测97例结肠癌组织标本及其对应正常结肠组织标本中Lgr5、E-cadherin、N—cadherin的表达情况。结果在结肠癌中,Lgr5在肿瘤组织中表达阳性率为55.7%(54/97),正常组织中Lgr5表达阳性率为5.2%(5/97)。Lgr5在肿瘤组织与正常组织中表达比较差异有统计学意义(X~2=54.480,P<0.05)。E-cadherin在肿瘤组织中表达阳性率为51.5%(50/97),正常组织中E-cadherin表达阳性率为87.6%(85/97)。E-cadherin在肿瘤组织与正常组织中表达差异具有统计学意义(X~2=29.837,P<0.05)。N-cadherin在肿瘤组织中表达阳性率为66%(64/97),正常组织中N-cadherin表达阳性率为15.5%(15/97),N-cadherin在肿瘤组织与正常组织中表达比较差异有统计学意义(X~2=51.271,P<0.05)。Lgr5的表达,结肠癌淋巴结有无转移比较差异有统计学意义(X~2=11.178,P<0.05),有无远处转移比较,差异有统计学意义(X~2=7.900,P<0.05)。Lgr5的表达和E-cadherin的表达呈负相关(r=-0.562,P<0.05),和N-cadherin的表达呈正相关(r=0.458,P<0.05)。结论 Lgr5表达水平的改变能够影响结肠癌细胞的侵袭转移能力,Lgr5可能促进结肠肿瘤细胞EMT的发生,参与了结肠癌的侵袭转移。
Objective To analyze the role of Lgr5 in the invasion and metastasis of colon cancer and its significance. Methods The expression of Lgr5, E-cadherin and N-cadherin in 97 specimens of colon cancer and their corresponding normal colorectal tissues were detected by immunohistochemistry. Results In colon cancer, the positive rate of Lgr5 was 55.7% (54/97) in tumor tissues and 5.2% (5/97) in normal tissues. The expression of Lgr5 in tumor tissue and normal tissue was significantly different (X ~ 2 = 54.480, P <0.05). The positive expression rate of E-cadherin in tumor tissue was 51.5% (50/97), while the positive expression rate of E-cadherin in normal tissue was 87.6% (85/97). E-cadherin in tumor tissue and normal tissue expression difference was statistically significant (X ~ 2 = 29.837, P <0.05). The positive expression rate of N-cadherin in tumor tissue was 66% (64/97), the positive expression rate of N-cadherin in normal tissue was 15.5% (15/97). The expression of N-cadherin in tumor tissue and normal tissue was different There was statistical significance (X ~ 2 = 51.271, P <0.05). Lgr5 expression, lymph node metastasis of colon cancer was significantly different (X ~ 2 = 11.178, P <0.05), with or without distant metastasis, the difference was statistically significant (X ~ 2 = 7.900, P <0.05 ). The expression of Lgr5 was negatively correlated with the expression of E-cadherin (r = -0.562, P <0.05), and positively correlated with the expression of N-cadherin (r = 0.458, P <0.05). Conclusion The change of Lgr5 expression can affect the invasion and metastasis of colon cancer cells. Lgr5 may promote the development of EMT in colon tumor cells and participate in the invasion and metastasis of colon cancer.