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目的:建立复方扶芳藤合剂中人参皂苷Rb1和黄芪甲苷的含量测定方法。方法:采用HPLC法,色谱柱为Shim-PackCLC-ODS柱(150 mm×6 mm,5μm);流动相为乙睛-水梯度洗脱;流速1.0mL.min-1;柱温为25℃;检测器为PL-ELS2100型蒸发光散射检测器(蒸发温度:115℃,雾化温度:80℃,气体流量:1.1 L·min-1)。结果:人参皂苷Rb1和黄芪甲苷分别在1.39～22.24μg,3.22～51.52μg范围内,其峰面积的自然对数(Y)与进样量的自然对数(X)呈良好的线性关系,r=0.999 5,r=0.999 3;平均加样回收率在97.3%～100.6%之间,RSD在1.1%～2.9%之间(n=9)。结论:该方法稳定、重复性好,结果准确,可作为复方扶芳藤合剂中人参皂苷Rb1和黄芪甲苷的含量测定方法。
Objective: To establish a method for the determination of ginsenoside Rb1 and astragaloside Ⅳ in Fufang Fufangtang Mixture. Methods: The column was Shim-Pack CLC-ODS (150 mm × 6 mm, 5 μm) by HPLC. The mobile phase was acetonitrile-water gradient. The flow rate was 1.0 mL.min-1. The column temperature was 25 ℃. The detector was a PL-ELS 2100 evaporative light scattering detector (evaporation temperature: 115 ° C, atomization temperature: 80 ° C, gas flow rate: 1.1 L · min -1). Results: Ginsenoside Rb1 and astragaloside were in the range of 1.39-22.24μg and 3.22-51.52μg, respectively. The natural logarithm of peak area (Y) showed a good linear relationship with the natural logarithm (X) of injection volume. r = 0.999 5, r = 0.999 3. The average recoveries ranged from 97.3% to 100.6% with RSD between 1.1% and 2.9% (n = 9). Conclusion: The method is stable, reproducible and accurate. It can be used as a method for the determination of ginsenoside Rb1 and astragaloside Ⅳ in Fufang Fufang Mixture.