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采用对虾白斑综合症病毒和传染性皮下组织坏死病毒的特异引物,在一个PCR反应中同时扩增到大小分别为271 bp和356 bp的病毒特异片段,多重PCR条件优化后可从最少100 fg级病毒DNA模板中定性检测出此两种病毒。
Using specific primers of WSSV and infectious subcutaneous tissue necrosis virus, the PCR-amplified 276 bp and 356 bp virus-specific fragments were amplified simultaneously from a minimum of 100 fg grade The two viruses were qualitatively detected in the viral DNA template.