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目的:建立RP-HPLC同时测定沙苁蓉中毛蕊花糖苷、金石蚕苷和2′-乙酰基金石蚕苷含量的方法。方法:采用Agi-lent TC-C18(250 mm×4.6 mm,5μm)色谱柱,柱温30℃;流动相为甲醇(A)-0.1%甲酸(B),梯度洗脱(0 min,35%A;30min,50%A),流速1.0 mL.min-1;检测波长330 nm。结果:在上述色谱条件下,毛蕊花糖苷、金石蚕苷和2′-乙酰基金石蚕苷获得良好分离;进样浓度分别在0.0214~0.856 mg.mL-1(r=0.9998)、0.1034~4.136 mg.mL-1(r=0.9999)、0.0996~3.984 mg.mL-1(r=0.9999)范围内呈良好的线性关系;平均加样回收率(n=6)分别为98.4%,101.0%,98.2%;精密度试验的RSD(n=6)小于0.33%;重复性试验的RSD(n=6)小于2.2%。结论:该方法简便、准确,重复性好,为沙苁蓉的质量控制提供可借鉴的分析方法。
OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of verbascoside, amelogenin and 2’-acetylcithin in Cistanche desertica. Methods: A column with Agi-lent TC-C18 (250 mm × 4.6 mm, 5 μm) was used and the column temperature was 30 ℃. The mobile phase consisted of methanol (A) A; 30min, 50% A), flow rate 1.0 mL.min-1; detection wavelength 330 nm. Results: Under the above chromatographic conditions, Verbascoside, Digoxin and 2’-Acetylsalicide were well separated. The injection concentrations were 0.0214 ~ 0.856 mg.mL-1 (r = 0.9998) and 0.1034 ~ 4.136 mg (r = 0.9999) and 0.0996 ~ 3.984 mg.mL-1 (r = 0.9999). The average recoveries were 98.4%, 101.0% and 98.2 %; RSD (n = 6) for precision test is less than 0.33%; RSD (n = 6) for repeatability test is less than 2.2%. Conclusion: The method is simple, accurate and reproducible, providing valuable analytical methods for the quality control of.