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目的：筛选并鉴定唐氏综合征胎儿与健康对照者差异性表达蛋白质，以寻找唐氏综合征胎儿的生物标记物。方法应用同位素标记相对和绝对蛋白定量法和功能聚类分析法筛查和分析唐氏综合征胎儿脐带血和正常胎儿脐带血的差异表达蛋白。结果共筛选鉴定到506种差异表达蛋白，其中最显著的3种蛋白分别是基质蛋白‐3、胸腺肽β10和骨桥蛋白。结论唐氏综合征胎儿和正常胎儿脐带血血清蛋白存在较多差异表达蛋白，其中基质蛋白‐3、胸腺肽β10和骨桥蛋白等显著差异蛋白可能作为潜在的临床筛查生物标志物。“,”Objective To identify the differentially expressed proteins between fetuses with Down′s syndrome and healthy control ,so as to screen the potential biomarkers of fetuses with Down′s syndrome .Methods To investi‐gate the differentially expressed proteins in cord blood of fetus with Down′s syndrome and normal fetus using isobaric tagging for relative and absolute protein quantification and gene gene ontology analysis .Results A total of 506 dif‐ferentiate proteins were identified ,in which 3 proteins (Matrin‐3 ,Thymosin beta 10 and Osteopontin) were signifi‐cant .Conclusion The proteomics of the serum are significant different among normal fetus and fetus with Down′s syndrome .Some of the protein ,such as Matrin‐3 ,Thymosin beta 10 and Osteopontin ,may have potential applying value in clinic screening pratice .