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细菌等原核细胞中,结构基因在基因组DNA上形成一个连续的编码区域,而真核细胞中的基因结构相对复杂,虽然同样分为编码区和非编码区,但真核基因的编码区域往往因非编码区即内含子穿插其中而使编码区分散。考查真核和原核基因结构差异的问题情境形式多样:例如涉及基因文库的建设和比较;DNA分子杂交技术应用中的问题分析以及基因表达过程中相关现象的解释等问题。这些问题一方面涉及的概念多,难辨析
Bacteria and other prokaryotic cells, the structural gene in the genomic DNA to form a continuous coding region, while the eukaryotic gene structure is relatively complex, although also divided into coding and non-coding regions, but the coding region of eukaryotic genes often due to Non-coding regions, ie introns interspersed with the coding region dispersed. For example, it involves the construction and comparison of gene libraries; the problems in the application of DNA molecular hybridization techniques and the interpretation of related phenomena in gene expression. On the one hand these issues involve many concepts, difficult to distinguish