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以金边墨兰无菌苗为试材，通过不同温度和不同植物生长调节剂对墨兰组培芽增殖和生长的影响研究表明：在试验范围内，在１／２ＭＳ固体培养基上，以６－ＢＡ２．０ｍｇ·Ｌ－１（单位下同）＋ＩＢＡ１．０、日温２８℃、夜温２４℃繁殖系数最高，以ＮＡＡ１．０或ＮＡＡ１．０＋ＩＢＡ１．０日温２６℃、夜温２２℃对根、茎、叶的生长和分化最为有利，二者结合可显著提高繁殖系数和幼苗生产量。
The results showed that: in the test range, on the 1/2 MS solid medium, the effect of different temperature and different plant growth regulators on the proliferation and growth of the tissue culture buds of Cymbidium -BA2.0mg · L-1 (unit same below) + IBA1.0, the daily temperature 28 ℃, night temperature 24 ℃ the highest reproductive coefficient NAA1.0 or NAA1.0 + IBA1.0 day 26 ℃, night temperature 22 ℃ The growth and differentiation of roots, stems and leaves are the most favorable. The combination of the two can significantly increase the breeding coefficient and seedling production.