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目的观察不同剂量硒喂饲SD大鼠后,对其肝、肾、血、睾丸中硒蓄积浓度的影响。方法将40只4周龄雄性断乳大鼠,以缺硒饲料(BD)喂饲35 d后,按体重随机分为4组,每组10只。1组继续BD饲养,其它3组的饲料在BD基础上分别加含0.25、3.0、5.0 ppm亚硒酸钠形式的硒。经过28 d硒蓄积性毒性试验,处死采样,测定肝、肾、血细胞(全血离心沉淀)和睾丸中硒元素蓄积浓度。结果适量硒摄入的大鼠肾与睾丸的硒浓度最高。高硒暴露时,组织硒浓度依次为:肾>肝>血细胞>睾丸。高浓度硒对4种组织硒浓度变化的影响依次是:血细胞>肝>肾>睾丸。结论肝与血细胞硒蓄积浓度的变动幅度大,提示它们对维持其它组织硒含量的相对稳定具有重要作用;而睾丸硒蓄积量的稳定性印证了该元素对雄性生殖功能的重要性。
Objective To observe the effects of different doses of selenium on SD rats after selenium feeding on selenium accumulation in liver, kidney, blood and testis. Methods Forty 4-week-old male weanling rats were fed with BD diet for 35 days. The rats were randomly divided into 4 groups according to body weight, with 10 rats in each group. Group 1 continued BD feeding, while other 3 groups fed selenium containing 0.25, 3.0, 5.0 ppm sodium selenite respectively on the basis of BD. After 28 days of selenium accumulation toxicity test, samples were sacrificed and liver, kidney, blood cells (whole blood centrifuge pellets) and testis selenium accumulation concentration were measured. Results Selenium intake of rat kidney and testicular selenium concentration highest. High selenium exposure, tissue selenium concentrations were: kidney> liver> blood cells> testis. The effects of high concentration selenium on the changes of selenium concentration in four kinds of tissues were: blood cells> liver> kidney> testis. Conclusions The changes of selenium concentration in liver and blood cells vary greatly, suggesting that they play an important role in maintaining the relative stability of selenium in other tissues. The stability of selenium accumulation in testis confirms the importance of this element to male reproductive function.