利用异氰酸-α萘酯(NI)作衍生化试剂,在Pirkle型DNBPG柱上拆分了β-阻断(?)丙洛尔等药物。方法简便,分离度好。对其衍生物结构的研究表明:在温和的条件下,异氰酸酯与分析物的仲氨基反应,生成脲;若反应在较强烈的条件下进行,则将发生异构化,最终是异氰酸酯与羟基结合,生成氨基甲酸酯。实验还表明,NI试剂与分析物之间的反应是按1:1进行的。 The α-naphthyl ester (NI) was used as a derivatization reagent to separate β-blockers (?) And other drugs on the Pirkle DNBPG column. Method is simple, good separation. Studies on the structure of their derivatives show that under mild conditions, the isocyanate reacts with the secondary amino group of the analyte to form urea. If the reaction is carried out under more vigorous conditions, isomerization will occur and eventually the isocyanate is bound to the hydroxyl group , Generate carbamate. Experiments also show that the reaction between NI reagents and analytes is performed at 1: 1.