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目的探讨催产素干预兔骨质疏松模型的骨髓脂肪变化规律及其可能机制,阐明磁共振波谱(MRS)动态评估的可行性。方法 40只8月龄雌兔,采用双侧去卵(OVX)法制作骨质疏松模型,随机分为骨质疏松模型组(A组,n=20)及催产素干预组(B组,n=20),后者OVX术后2周皮下注射催产素,持续4周。两组于OVX术后3、6、10、14周(每个时间点5只兔)行左股骨上段MRS扫描,计测骨髓脂肪分数(FF)值;同时,采集兔血清测定雌激素(E)、总钙(Ca)、抗酒石酸酸性磷酸酶(TRAP)、特异性碱性磷酸酶(BALP-B)水平。结果 A、B组实验兔骨髓FF值随时间呈渐进性增高,两组每个时间点分别为(46.20±5.24)%、(64.39±5.53)%、(69.13±6.14)%、(78.14±7.42)%以及(42.8±7.11)%、(52.00±3.77)%、(58.25±3.75)%、(61.5±6.63)%,B组自第6周始FF值低于A组,TRAP自6周起低于A组,BALP-B自10周开始高于A组,差异有统计学意义(P<0.01)。两组雌激素、总钙水平随时间变化均有所降低。结论催产素早期干预骨质疏松模型兔能降低骨髓脂肪的堆积,影响骨质疏松的发展进程,早期可能通过抑制破骨、后期通过促进成骨发生作用,MRS可以对此过程进行动态评估。
Objective To investigate the changes of bone marrow adiposity induced by oxytocin in rabbit model of osteoporosis and its possible mechanism, and to clarify the feasibility of magnetic resonance spectroscopy (MRS) dynamic assessment. Methods Forty eight-month-old female rabbits were randomly divided into three groups: osteoporosis model group (n = 20) and oxytocin intervention group (n = = 20), the latter OVX 2 weeks after the oxytocin subcutaneous injection for 4 weeks. The left femur MRS scan was performed at 3, 6, 10, and 14 weeks after OVX operation (5 rabbits at each time point), and the bone marrow fat fraction (FF) was measured. Meanwhile, the serum levels of estrogen ), Total calcium (Ca), tartrate-resistant acid phosphatase (TRAP), and specific alkaline phosphatase (BALP-B) levels. Results The FF value of bone marrow of experimental rabbits in group A and group B gradually increased with time, and the values of (46.20 ± 5.24)%, (64.39 ± 5.53)%, (69.13 ± 6.14)%, (78.14 ± 7.42) ), (42.8 ± 7.11)%, (52.00 ± 3.77)%, (58.25 ± 3.75)%, (61.5 ± 6.63)% respectively in group B. The FF value of group B was lower than that of group A Lower than group A, BALP-B was higher than group A from the 10th week, the difference was statistically significant (P <0.01). Both estrogen and total calcium levels decreased over time. Conclusions Early intervention of oxytocin in osteoporosis model can reduce the accumulation of bone marrow adipose tissue and affect the development of osteoporosis. Early inhibition of osteoclasts may lead to osteogenesis in the early stage, and osteogenesis may be promoted in later stage. MRS can evaluate this process dynamically.