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目的为了解嗅刺激引起中枢神经系统兴奋的区域及程度。方法以醋酸戊酯多次刺激大鼠，１ｈ后灌注固定处死动物。以ｃ－ｆｏｓ探针的ＡＢＣ免疫细胞化学法，观察分析ｃ－Ｆｏｓ蛋白在大鼠脑内标记区。结果中枢系统内ｃ－Ｆｏｓ蛋白标记区十分广泛，尤以脑桥以上脑区为多。除嗅觉通路诸结构外，边缘系统内也有广泛标记，提示存在嗅情绪反应、嗅内脏与嗅躯体反应及嗅记忆等多神经回路的活动。其次，与注意及中枢功能状态调节有关的脑区也有广泛标记。上丘深层、下丘及脑桥核等为强标记，推测与感觉和运动的整合活动有关。结论用嗅刺激诱导ｃ－ｆｏｓ原癌基因表达，确可证实一种特异性嗅刺激可引起大鼠中枢神经系统内泛脑网络上的广泛运动。
Purpose To understand the area and extent of excitation of the central nervous system caused by olfactory stimuli. Methods Rats were stimulated with amyl acetate for several times and animals were perfused and fixed after 1h. ABC immunocytochemistry with c-fos probe was used to observe the labeling region of c-Fos protein in rat brain. Results The c-Fos protein labeling region in the central nervous system was very extensive, especially in the brain regions above the pons. In addition to the structure of the olfactory pathway, the limbic system also has a wide range of markers, suggesting the existence of sniffing emotional reactions, sniffing visceral and olfactory somatic responses and olfactory memory and other multi-nerve circuits. Second, brain regions associated with attention and central functional status are also widely marked. Deep on the hill, the inferior colliculus and pons and nucleus as a strong marker, speculated that sensory and motor integration activities. Conclusion Olfactory stimulation induces the expression of c-fos proto-oncogene, confirming that a specific olfactory stimulus can induce extensive motor activity in the pannus network in the central nervous system of rats.