目的 :观察应激对动物生殖内分泌的影响 ,建立符合临床需要的生殖内分泌紊乱的动物模型。方法 :用悬吊和制动方法造成动物急性或重复应激状态 ,用放射免疫结合的方法测定血浆有关性激素的水平 ,用阴道涂片的方法观察雌性动物的性周期 ,用常规方法观察雄性动物的性行为。结果 :急性制动应激和急性悬吊应激 1,2 ,3h均能使雄性小鼠血浆睾酮水平显著降低 ,皮质酮水平则明显升高。大鼠急性制动应激 3h ,可见血浆睾酮水平及睾丸中合成酶 17α 羟化酶、17,2 0 羟甾脱氢酶和 3 β 裂解酶活性显著降低 ,皮质酮水平则明显升高。重复悬吊应激可使雄性小鼠性行为低下或缺损 ,雌性小鼠性周期紊乱 ,分别可见血浆睾酮和雌二醇水平降低。结论 :应激可使生殖内分泌功能低下或紊乱 ,在此过程中性激素降低起着重要的作用。提示临床上各种躯体或心理应激引起的生殖内分泌功能低下或紊乱也与相关性激素降低或分泌节律失常有关 ,应激性生殖内分泌功能低下或紊乱动物模型可供相关药物研究 Objective: To observe the impact of stress on animal reproductive and endocrine, and to establish an animal model of reproductive and endocrine disorders in line with clinical needs. Methods: Acute or repeated stress state was induced by suspension and braking. Plasma levels of sex hormones were measured by radioimmunoassay. Sexual cycles of female animals were observed by vaginal smear method. Male animals were observed by conventional methods Sexual behavior. Results: Acute immobility stress and acute stress for 1, 2 and 3 h reduced plasma testosterone levels and corticosterone levels in male mice significantly. Rat acute braking stress 3h, showing that plasma testosterone levels and testis in the synthase 17α hydroxylase, 17,2 0-hydroxy-desaturase and 3 β-lyase activity was significantly reduced, corticosterone levels were significantly higher. Repeated suspension stress can make male mice with low or defective sexual behavior, female mice sexual cycle disorder, respectively, decreased plasma testosterone and estradiol levels. CONCLUSIONS: Stress can make reproductive endocrine dysfunction or disorder, in the process of reducing sex hormone plays an important role. Suggesting that clinically various physical or psychological stress caused by reproductive endocrine dysfunction or disorder associated with reduced or secreted hormonal rhythm disorders related to stress genitourinary endocrine dysfunction or disorders in animal models for relevant studies