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目的 研究细胞周期调控蛋白在温石棉所致人胚肺成纤维细胞 (HEPF)增殖中的表达和蛋白激酶C(PKC)对细胞周期调控蛋白的影响。方法 利用体外细胞培养技术 ,用流式细胞仪测定温石棉介导的HEPF的细胞周期调控蛋白的表达 ,同时设立阳性对照 (标准SiO2 )、阴性对照 (标准TiO2 )、正常对照 (未经粉尘处理的兔肺泡巨噬细胞 )和空白对照。结果 温石棉组细胞周期蛋白 (cyclin)CyclinD1、CyclinE、增殖细胞核抗原 (PCNA)和CyclinA表达阳性率分别为 19.0 %、2 2 .8%、79.7%和 6 5 .1% ,均较各对照组低 ,差异有显著性 (P <0 .0 5 ) ,且CyclinD1和CyclinE表达阳性率低于PCNA和CyclinA ,差异有显著性 (P <0 .0 1) ;而CyclinB1和p34cdc2激酶的阳性表达率较空白对照和正常对照略高。当PKC信号通路被阻断后 ,PCNA在温石棉组表达的阳性率明显降低 ,差异有显著性 (P <0 .0 5 ) ,p34cdc2激酶的阳性表达率改变不明显。结论 这几种蛋白可能均参与了温石棉所致HEPF增殖 ,其中PCNA表达的改变可能与上游PKC信号通路有关
Objective To investigate the expression of cell cycle regulatory proteins (HSPCs) in human choriocarcinoma-induced human embryonic lung fibroblasts (HEPF) proliferation and the effect of protein kinase C (PKC) on cell cycle regulatory proteins. Methods The cell cycle regulatory protein of HEPF was detected by flow cytometry in vitro. The positive control (standard SiO2), negative control (standard TiO2) and normal control (without dust treatment) Rabbit alveolar macrophages) and blank control. Results The positive rates of CyclinD1, CyclinE, PCNA and CyclinA in chrysotile were 19.0%, 22.8%, 79.7% and 65.1% respectively, which were significantly higher than those in control group (P <0.05), and the positive rate of CyclinD1 and CyclinE expression was lower than that of PCNA and CyclinA (P <0.01), while the positive expression rate of CyclinB1 and p34cdc2 kinase Slightly higher than the control and normal controls. When the PKC signal pathway was blocked, the positive rate of PCNA expression in chrysotile significantly decreased, the difference was significant (P <0. 05), p34cdc2 kinase positive expression rate was not significantly changed. Conclusion These proteins may participate in the proliferation of HEPF induced by chrysotile, and the change of PCNA expression may be related to the upstream PKC signaling pathway